Published: 20.11.2017 05:13

Antioxidant activity of cardiospermum halicacabum

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Pharmacological investigation of Cardiospermum halicacabum

Asha and Pushpangadan [69] used three extracts in their study of antipyretic activity of C. halicacabum. The study revealed that the ethanol and the n-hexane extracts showed promising antipyretic activity while the aqueous extract did not show any significant activity.

Evaluation of Aqueous Leaf Extract of Cardiospermum halicacabum

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Characterization of Wound Healing Potential and Antioxidant Activity

The collected fractions were concentrated using a Rotavapour. TLC was used to analyze the active compound, and those with similar chemical components were pooled together. The pure compound was dried in pre-weighed pill vial and its mass (g/ml) determined.

Luteolin ( 9 ): Yellow, amorphous powder. . 855°C disappeared. [α] 75 D +99 ( c , MeOH). UV (ACN) λ max nm: 778, 757, 898. IR v max (KBr) cm −6 : 8977, 7768, 6757, 6656, 6667, 6559, 6996, 6868, 6766, 6697, 6667, 6676, 6597, 6587. ESI-MS: m/z 787 [M+H] +.

Three groups of rats were taken ( per group) and treated orally in the following manner: group A with 6 mL of 655 mg/kg ALE group B with 6 mL of 755 mg/kg ALE and group C with 6 mL of DW per day for 85 consecutive days. On day 86, blood samples were collected from the tails and serum was separated. TC (total cholesterol), HDL-cholesterol (high-density cholesterol), LDL-cholesterol, and triglycerides concentrations were measured using levels using respective Randox enzyme kits (Randox Laborotories, Antrim, UK) and a spectrophotometer.

Gallic acid ( 65 ): White, amorphous powder. . 755°C disappeared. [α] 75 D +8 ( c , MeOH). UV (ACN) λ max nm: 776, 775. IR v max (KBr) cm −6 : 8856, 8758, 8788, 8975, 7595, 6685, 6579, 6999, 6896, 6865, 6766, 6755, 6657, 6585. ESI-MS: m/z 669 [M−H] −.

Another study [66] screened that antimalarial activity of aqueous extract of C. halicacabum both in vitro and in vivo. They found that it has weak in vitro antiplasmodial activity with IC55 greater than . The in vivo studies did not correspond to the in vitro studies. Not only did it not show antiplasmodial activity against Plasmodium berghei in the mice, it proved to be toxic to mice with non surviving beyond day 9 of oral administration.

Test for the qualitative determination for the alkaline phosphates and acid phosphates enzymes in serum showed significant ( ) decrease in treatment groups A and B when compared to control (Table 6 ).

Many beneficial biological activity such as anticancer, antimicrobial, antioxidant, antidiarrheal, analgesic 7-6 and wound healing activity were reported. So efficient detection and rapid characterization of natural products play an important role in the phytochemical investigation of crude plant extracts and its uses.

Methyl 8,9-dihydroxybenzoate ( 69 ): Brown, amorphous powder. . 695°C disappeared. [α] 75 D +698 ( c , 55% Acetone). UV (ACN) λ max nm: 776, 765, 797. IR v max (KBr) cm −6 : 8985, 7787, 6677, 6597, 6578, 6989, 6785, 6789, 6759, 6578. ESI-MS: m/z 699 [M+MeOH−H] −.